| Step | Reagent | Time |
|---|---|---|
| Deparaffinization | 100% xylene | 5 min |
| 100% xylene | 5 min | |
| Rehydration | 100% ethanol | 3 min |
| 100% ethanol | 3 min | |
| 95% ethanol | 3 min | |
| 80% ethanol | 1 min | |
| water | 5 min | |
| Hematoxylin staining | hematoxylin | 1 min |
| water | 3 min | |
| Differentiation | 0.5% hydrochloric acid in 70% ethanol | 10 s |
| water | 5 min | |
| Eosin staining | 95% ethanol | 10 s |
| eosin | 45 s | |
| Dehydration | 95% ethanol | 10 s |
| 95% ethanol | 10 s | |
| 100% ethanol | 1 min | |
| 100% ethanol | 1 min | |
| Clearing | 100% xylene | 1 min |
| 100% xylene | 1 min |
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