Figure 3. Ccr5^−/− mice have increased inflammatory responses to systemic C. albicans infection. WT and Ccr5^−/− mice were intravenously injected with 3×10⁵ CFUs of C. albicans. (A-D) Levels of IL-6 (A), TNF-α (B), CXCL1 (C), and CXCL2 (D) in kidney lysates at day 3 post-infection (n=4 or 6 kidneys/group). (E, F) Kidney cells were isolated, and stained with anti-CD45, anti-CD11b, and anti-Ly6G Abs. (E) Representative FACS plots for CD45⁺ leukocytes and their percentages and absolute numbers were presented (n=4 or 6 kidneys/group). (F) CD45⁺ leukocytes were gated and FACS plots for CD11b⁺ and Ly6G⁺ neutrophils and their percentages in leukocytes were presented (n=4 or 6 kidneys/group). (G, H) Serum creatinine and BUN levels (n=3 or 4 mice/group). Two-way ANOVA test was used. Results are representative of 2–4 experiments and are presented as the mean±SEM.

^*p<0.05; ^**p<0.01; ^***p<0.001.