Figure 7. GM-CSF enhances IL-23 production by DCs. (A-D) Kideys were harvested at day 3 post-infection and their expression of IL-23 was investigated. (A, B) Transcriptional levels of Il23a and Il12b (n=4 or 5 kidneys/group). (C) Concentrations of renal IL-23 protein (n=5 or 6 kidneys/group). (D) FACS hitograms for IL-23A intracellular staining in renal CD45⁺MHCII⁺CD11c⁺ DCs and percentages of IL-23⁺ cells. Red line: WT; blue line: CCR5^−/− (n=5 kidneys/group). (E) BMDCs were cultured with combination of GM-CSF and hyphae forms of heat-kille (HK) C. albicans for 24 h. Concentrations of IL-23 in culture supernantants were determined using ELISA (n=3/group). (F) The percentage of CD45⁺MHCII⁺CD11c⁺ DCs in naïve mice (n=3 kidneys/group). Results are representative of 2–3 experiments and are presented as the mean±SEM.

^*p<0.05; ^**p<0.01; ^***p<0.001.