FIGURE 2.

Eomes knockdown led to reduced proliferation of esophageal cancer cells in vitro. A, Different expression levels of Eomes mRNA in different esophageal cancer cell lines. B, The knockdown efficiency of Eomes was detected by qRT‐PCR in KYSE450 and TE7 cell lines. C, D, Effects of Eomes knockdown on colony formation in esophageal cancer cells. E, Effects of Eomes knockdown on the esophageal cancer cells cycle by flow cytometry. F, Effects of Eomes knockdown on the proliferation of esophageal cancer cells by CCK8. G, CFSE staining to detect the effect of Eomes knockdown on the proliferation of esophageal cancer cells. H, Effects of Eomes knockdown on apoptosis of esophageal cancer cells by flow cytometry. Mean ± SD of relative fold changes from triplicate experiments was plotted. *P < .05, **P < .01, ***P < .001