Skip to main content
. 2020 Nov 10;112(1):217–230. doi: 10.1111/cas.14700

Figure 1.

Figure 1

Collagen films induce cancer stem cell (CSC) enrichment. A, Morphology of LT73 cancer cells grown on culture plates, pristine collagen matrices and Glc‐collagen and Gal‐collagen matrices respectively (72 h). B, FACS analysis for the expression of CD133 and CXCR4 in A549, LT73 and H460 cell lines cultured on plastic culture plates (Control), pristine and Glyco‐collagens for 72 h. Mean ± SEM of n = 4 experiments are shown. One‐way ANOVA was used for statistical analyses; ***P < 0.001, **P < 0.01. C, Relative expression of Oct4/Nanog stemness genes and (D) ABCG2 drug transporter in A549, LT73 and H460 cell lines cultured on different collagen films for 72 h. Cells cultured on plastic culture plates were used as calibrator. Bars represent the mean fold‐change of n = 3 independent experiments. ANOVA was used for statistical analyses; *P < 0.05; **<0.01