Figure 4.

Expression of activated integrin β1 correlates with tumor cell binding to Glc‐collagen matrices. A, FACS analysis of the membrane expression of the activated form of integrin β1 (clone 9EG7) alone (left panel) and in co‐expression with integrin alpha1 (central panel) or alpha2 subunits (right panel) in A549, LT73 and H460 cell lines, both in the bulk population and within the gated CD133+ subset. Bars are the mean ± SEM of n = 4 replicates for each cell line. ANOVA was used for statistical analysis. *P < 0.05; **P < 0.001. B, Time‐course analysis of A549, LT73 and H460 cell line adhesion to culture plates, collagen and Glc‐collagen matrices. At each indicated time point, adherent cells were detached and counted by the Trypan blue exclusion method. Two‐way ANOVA was used for statistical analyses. *P < 0.05; *** P < 0.0001