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. 2020 Nov 10;112(1):217–230. doi: 10.1111/cas.14700

Figure 6.

Figure 6

Integrin β1 mediated collagen‐induced cancer stem cell (CSC) enrichment. A, Time‐course analysis for modulation of CD133+ cells and integrin β1 active form during A549, LT73 and H460 cell line adhesion to pristine and neoglucosylated collagen films. Fold‐change of positive cells relative to culture plates are reported after 30 min, 4 h and 24 h post cell seeding on films. n = 3 replicates/time point for each cell line. Two‐way ANOVA was used for statistical analyses, **P < 0.01; ***P < 0.001. B, Time‐course analysis of modulation of CD133+ cells during adhesion to collagen films of A549, LT73 and H460 cell lines, transfected with scrambled siRNA or siITGB1.Fold‐change in the percentage of CD133+ positive cells relative to culture plates are reported after 4 h and 24 h post cell seeding on films. n = 2 experiments for each cell line. Two‐way ANOVA was used for statistical analyses. **P < 0.01. C, FACS analysis for CD133 expression in cancer cell lines cultured for 4 h on collagen matrices or culture plate in presence of integrin β1 blocking antibody (50 μg/mL). Bars are the mean value ± SD of n = 2 independent experiments performed for each cell line, in technical duplicate. ANOVA was used for statistical analyses. *P < 0.05