FIGURE 1.

Expression and clinical correlation of URB1 in colorectal cancer (CRC). A, Comparison of URB1 expression in CRC tissues and adjacent normal tissues based on The Cancer Genome Atlas (TCGA) datasets. COAD, colon adenocarcinoma; READ, rectal adenocarcinoma. B, mRNA expression of URB1 was measured in paired clinical CRC samples using quantitative real‐time PCR (n = 40). C‐E, Immunohistochemistry (IHC) was utilized to measure the quantification of URB1 protein expression in clinical CRC tissue microarray (HcolA180Su17) (n = 101). F, Correlation between URB1 expression and K‐RAS status from CRC tissue microarray (HcolA160Cs01). G, Overexpression of URB1 was associated with greater T stage (P = .012) and TNM stage (P = .005) in CRC patients. H, Quantification and representative photomicrographs of IHC staining for URB1 protein expression in TMA of CRC. Scale bar = 100 μm (×40 magnification) and 50 μm (×400 magnification). Data are expressed as mean ± SD. *P < .05, **P < .01. ns, not significant