FIGURE 2.

Silencing of URB1 inhibits proliferation and induces apoptosis in colorectal cancer (CRC) cells. A, URB1 mRNA and protein expression in a normal human colon epithelial cell line (NCM460) and the human CRC cell lines (LoVo, RKO, HCT116, and SW480) were assessed by quantitative real‐time PCR (qRT‐PCR) and western blotting. B, Interference efficiency of two specific shRNAs (sh1 and sh2) to URB1 in CRC cells was measured by qRT‐PCR and western blotting. C, D, Celigo cell counting assays and MTT assays indicated that URB1 silencing inhibited proliferation in CRC cells. E, Colony formation in CRC cells was repressed by URB1 silencing. F, Apoptosis in CRC cells was increased by URB1 silencing. Scale bar = 50 μm (×100 magnification). Data are expressed as mean ± SD (n = 3). **P < .01, ***P < .001, ****P < .0001. nc, control lentiviral vector; ns, not significant