FIGURE 6.

URB1 overexpression promotes proliferation in colorectal cancer (CRC) cells and fibroblasts. A, URB1 overexpression efficiency in CRC cells was measured by quantitative RT‐PCR and western blotting. B‐D, MTT, colony formation, and flow cytometry (FCM) assays were used to evaluate the effect of URB1 overexpression on CRC cells. E, URB1 overexpression efficiency in fibroblasts assessed by western blotting. F, G, FCM and MTT assays indicated that URB1 overexpression promotes cell cycle progression and proliferation in fibroblasts. Blank, blank control group; URB1, URB1 overexpression group; Vector, negative control group. H, Regulation of URB1 on X‐box binding protein 1 (XBP1) and activating transcription factor 4 (ATF4) in CRC cells was measured by western blotting. Data are expressed as mean ± SD (n = 3). *P < .05, **P < .01, ***P < .001, ****P < .0001. ns, not significant