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. 2020 Nov 18;112(1):155–167. doi: 10.1111/cas.14670

FIGURE 5.

FIGURE 5

Roles of β2‐adrenergic receptor in isoxsuprine‐induced inhibition of mesenchymal traits. The control (wild type: WT) and β2‐AR gene (ADRB2) knockout (ADRB2 KO #1) SAS cells were cultured in the absence (−/control) or presence of 10 μmol/L isoxsuprine for 72 h, followed by the qRT‐PCR analyses for the expression of IL‐6 (A), vimentin (B), N‐cadherin (C), fluorescence immunostaining (D) for vimentin (red) and nuclei (blue) and quantification of vimentin‐positive area (E). Scale bar: 100 μm. F, G, WT and ADRB2 KO #1 cells were cultured in the absence (−/control) or presence of 10 μmol/L isoxsuprine for 72 h. The cells migrated for 48 h were stained (F) and counted (G). Scale bar: 200 μm. Data are represented as mean ± SD **P < 0.01 ***P < 0.001; ns, not significant