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. 2020 Nov 29;112(1):265–274. doi: 10.1111/cas.14724

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© 2020 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Irradiated supernatant (SR) promotes hepatocellular carcinoma (HCC) cell migration and invasion. A, The effects of nonirradiated (SnonR), irradiated (SR), and irradiated plus drug (celecoxib)‐treated S(R + D) supernatant on RH6Gy cell migration were examined by wound healing assays, and wound healing was quantified as the ratio of the remaining cell‐free area to the area of the initial wound (calculated as the mean percentage) using ImageJ software. B, The effects of SnonR, SR, and S(R + D) on RH6Gy cell invasion were examined by transwell invasion assays (N = 3). Quantitative data are presented as the mean ± standard deviation. *P < .05, **P < .01