TABLE 2.
Extracted DNA/RNA and success rate of next‐generation sequencing (NGS) according to biopsy methods
| All | Cytology sample (N = 153) | Tissue sample (N = 69) | |||||
|---|---|---|---|---|---|---|---|
| EBUS‐GS‐TBB (bronchial brushing) | EBUS‐TBNA (needle washing) | Pleural effusion | Thoracoscopy | CNB | Surgical biopsy | ||
| Extracted yield of nucleic acid (ng/mL) | |||||||
| DNA median (range) | 90 (10‐400) | 70 (10‐380) | 90 (10‐400) | 270 (110‐330) | 190 (30‐350) | 70 (10‐380) | 215 (100‐350) |
| RNA median (range) | 20 (10‐790) | 10 (10‐80) | 20 (10‐290) | 50 (10‐680) | 55 (10‐340) | 10 (10‐210) | 260 (10‐790) |
| NGS success rate (95% CI) | |||||||
| DNA sequencing |
99.1% (96.8‐99.9) (220/222) |
98.7% (95.4‐98.8) (P = 0.999 a ) |
100% (95.8‐100) |
||||
|
97.9% (92.7‐99.7) (P = 0.999 b ) |
100% (93.6‐100) (P = 1.00 b ) |
100% (76.2‐100) (P = 1.00 b ) |
100% (82.9‐100) (P = 1.00b) |
100% (92.2‐100) (P = 1.00b) |
100% (82.9‐100) |
||
| RNA sequencing |
85.1% (79.8‐89.5) (189/222) |
83.0% (76.1‐88.6) (P = 0.224 a ) |
89.9% (80.2‐95.8) |
||||
|
84.4% (70.5‐93.5) (P = 0.174 b ) |
80.4% (71.1‐87.8) (P = 0.069 b ) |
100% (76.2‐100) (P = 1.00 b ) |
100% (82.9‐100) (P = 1.00 b ) |
81.1% (64.8‐92.0) (P = 0.089 b ) |
100% (82.9‐100) |
||
Abbreviations: CNB, core needle biopsy; EBUS‐GS‐TBB, transbronchial biopsy using endobronchial ultrasound sonography guide sheath; EBUS‐TBNA, endobronchial ultrasound sonography–guided needle aspiration biopsy.
Success rate of cytology sample (N = 153) was compared with tissue sample (N = 69).
Success rate of each diagnostic modality was compared with surgical biopsy.