Fig 4.

Arbutin mediates the regulatory action of miR‐338‐3p/MTHFD1L in OS cells. (A) The expression of miR‐338‐3p in Arbutin‐treated OS cells. (B) Transwell migration and invasion experiments were implemented in Arbutin‐treated MG‐63 and SW1353 cells after the transfection of si‐MTHFD1L, miR‐338‐3p inhibitor and si‐MTHFD1L + miR‐338‐3p. (C) CCK‐8 assay was conducted in Arbutin‐stimulated MG‐63 and SW1353 cells transfected with si‐MTHFD1L or miR‐338‐3p inhibitor. Scale bars, 200 μm. Data are shown as the means ± SD of three independent experiments. The data were statistically analyzed by Student’s t‐test or Dunn’s test. **P < 0.01 versus control or NC group; ^## P < 0.01 versus Arbutin group; ⁺⁺ P < 0.01 versus si‐MTHFD1L or miR‐338‐3p inhibitor group.