Skip to main content
. 2020 Dec 14;9(12):e1223. doi: 10.1002/cti2.1223

Figure 4.

Figure 4

The impact of α‐CD3/C28 bead ratios on iTreg stability upon re‐stimulation. Following a 7‐day stimulation and a 3‐day rest, cells were re‐stimulated using a 1:1 α‐CD3/CD28 beads and IL‐2 without iTreg differentiation factors for 7 days then rested with IL‐2 for 3 days. After 3 days of rest, (a) expression of FOXP3 and CD25 (b) suppressive activities were evaluated. MFI of FOXP3 and CD25 were normalised to highest raw MFI value in each experiment and represented as nMFI (%). Raw MFI for (a) is shown in Supplementary figure 12. For (b), grey‐shaded histogram represents positive control (no Treg control). Data are represented as mean ± sem, N = 3 in three independent experiments. For each donor (N), technical triplicates were utilised, and the average of technical replicates was used for each datapoint. Statistical significance identified by RM one‐way (a) and RM two‐way (b) ANOVA with Dunnett’s multiple comparisons test: *P < 0.05, **0.01, ***0.001, ****0.0001.