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. 2020 Dec 1;11(1):251–264. doi: 10.1002/2211-5463.13015

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© 2020 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig. 4 — (A) The expression of the Hippo signal pathway‐related proteins in differently treated CSC‐MCF‐7 cells (Blank, pcDNA3.1‐SOX21‐AS1, pcDNA3.1, si‐SOX21‐AS1#1, si‐SOX21‐AS1#2, si‐NC) was detected by western blot analysis. (B) The ratio of CD44⁺/CD24^–in CSC‐MCF‐7 cells after different treatments (Blank, pcDNA3.1‐SOX21‐AS1 + control, pcDNA3.1 + control, pcDNA3.1‐SOX21‐AS1 + Hippo activator, pcDNA3.1 + Hippo activator) was detected by flow cytometer analysis. (C) The expression of CSC‐MCF‐7 cell stemness‐related proteins after different treatments (Blank, pcDNA3.1‐SOX21‐AS1 + control, pcDNA3.1 + control, pcDNA3.1‐SOX21‐AS1 + Hippo activator, pcDNA3.1 + Hippo activator) was detected by western blot analysis. (D) The proportion of SP in CSC‐MCF‐7 cells after different treatments (Blank, pcDNA3.1‐SOX21‐AS1 + control, pcDNA3.1 + control, pcDNA3.1‐SOX21‐AS1 + Hippo activator, pcDNA3.1 + Hippo activator) was detected by flow cytometer analysis. (E) Sphere formation ability in CSC‐MCF‐7 cells after different treatments (Blank, pcDNA3.1‐SOX21‐AS1 + control, pcDNA3.1 + control, pcDNA3.1‐SOX21‐AS1 + Hippo activator, pcDNA3.1 + Hippo activator) was detected using a stereomicroscope. Scale bars = 50 μm (n = 3). The results are reported as the mean ± SD of three experiments. *P < 0.05; **P < 0.01 (Student′st‐test).