Figure EV3. ChHFR1 and AtHFR1 complement the Arabidopsis thaliana hfr1‐5 mutant long hypocotyl phenotype.
- Cartoon of HFR1 promoters from A. thaliana (pAtHFR1) and C. hirsuta (pChHFR1). These promoters cover 2,000 bp from the beginning of the translation start of the two HFR1 genes. The positions of G‐boxes (CACGTG) are indicated with arrows.
- GUS staining of representative A. thaliana seedlings expressing GUS under the pAtHFR1 (line #03). Seven‐day‐old W‐grown seedlings were treated with W + FR for the indicated amount of time.
- Correlation between HypW+FR‐HypW (means ± SE of at least four biological replicates, data shown in Fig 3C) and relative levels of ChHFR1 or AtHFR1 expression (means ± SE of three biological replicates, data shown in Fig 3B). The estimated regression equations and the R 2 values are shown for each plot.