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. 2020 Dec 2;40(1):e104273. doi: 10.15252/embj.2019104273

Figure 6. AtHFR1 interacts with AtPIF7.

Figure 6

  • A
    Y2H growth assay showing the interaction between AtHFR1 and AtPIF7. The BD‐ and the AD‐derivative constructs used in the assay are shown on the left side of the panel. SD‐LW or SD‐HLW refer to the selective medium (plated as drops in dilutions of 1, 1:10, and 1:100) indicative of transformed cells or interaction between the hybrid proteins, respectively. Truncated forms of murine p53 (BD‐fused) and SV40 large T‐antigen (AD‐fused), known to interact, were used as a positive control. Empty vectors (/) were used as negative controls.
  • B, C
    Hypocotyl length of seedlings of AtWT, (B) pif7‐1, hfr1‐5, pif7‐1 hfr1‐5 (top graph), pif7‐2, hfr1‐5, and pif7‐2 hfr1‐5 (bottom graph) mutants, and (C) transgenic 35S:GFP‐ΔNt‐HFR1 (35S:ΔNt‐HFR1), two lines of 35S:PIF7‐CFP (35S:PIF7 #1 and #2), and 35S:GFP‐ΔNt‐HFR1 35S:PIF7‐CFP double transgenic (35S:ΔNt‐HFR1 × 35S:PIF7 #1 and #2) seedlings grown under different R:FR. Seedlings were grown in W (R:FR > 1.5) for 7 days or for 2 days in W and then transferred to two W + FR treatments (R:FR 0.06 or 0.02) for 5 additional days. Values of hypocotyl length are the means ± SE of three independent biological replicates (at least 10 seedlings per replica).
  • D
    Aspect of representative 7‐day‐old W‐grown seedlings shown in C. Scale bar is 1 cm.