Figure EV3. Characterization of DNA in H2A.B‐NCP .

• A, B
  Protection of H2A.B‐NCP DNA against MNase digestion. The H2A.B nucleosomes containing a 147‐bp Widom 601 sequence DNA were treated with increasing amounts of MNase at 30°C. After 30 min incubation, the reactions were quenched by adding EGTA and proteinase K. The protected DNA was extracted and analyzed by 10% Native‐PAGE with ethidium bromide staining (A). Length of DNA protected against MNase digestion. A standard curve is prepared by plotting the length of each DNA standard versus the mobility measured from the EMSA. The length of the 147‐bp DNA, 118‐bp DNA, and 103‐bp DNA are then calculated from the standard curve (B).