Figure 6. DNA length dependence of the topological effects of Smc5/6 complex inactivation.

• A–D
  DNA topology of the indicated minichromosomes of increasing DNA length (kb) before (26°C) and after inactivation of Smc5/6 complex (35°C) in smc6‐9 cells. In each case, the first 2D gel resolves the Lk topoisomers (Lk), and the second 2D gel uncovers the knotted forms (Kn). Gel signals are denoted as in Fig 2. Bottom graphs compare the P^kn before and after the inactivation of Smc5/6 complex (mean ± SD from two independent experiments).
• E
  Plot of P^kn values of minichromosomes of increasing DNA length (including YEp13) before and after inactivation of Smc5/6 complex.