Figure EV3. Ablation of the mitotic surveillance pathway restores brain size and increases survival but does not rescue cilia defects in Sas4^cKO animals. Related to Fig 3 .

• A
  Telencephalon area of P60 brains of the indicated genotypes. WT littermates N = 6, Trp53bp1 ^−/− N = 5, Usp28 ^−/− N = 5, Trp53 ^−/− N = 4; one‐way ANOVA with post hoc analysis. 4 WT data points are from Fig 1B and shown alongside for comparison.
• B
  Cortical thickness of P60 brains of the indicated genotypes. WT littermates N = 4, Trp53bp1 ^−/− N = 3, Usp28 ^−/− N = 4, Trp53 ^−/− N = 3; one‐way ANOVA with post hoc analysis. WT data are from Fig EV1B and shown alongside for comparison.
• C
  Representative histology images of P14 brains of the indicated genotypes. Arrows indicate the enlarged ventricles caused by the lack of motile cilia. Scale bar = 0.2 cm.
• D
  Kaplan–Meier survival analysis of Sas4^cKO, Sas4^cKO;Trp53bp1 ^−/−, Sas4^cKO;Usp28^cKO, Sas4^cKO;Trp53 ^−/− animals compared to WT littermates. P values were calculated using the log‐rank test.
• E
  Quantification of total number of cells within a 500 μm‐width column of cortices at P14. WT littermates N = 4, Sas4^cKO N = 3, Sas4^cKO;Trp53bp1 ^−/− N = 4, Sas4^cKO;Usp28^cKO N = 5, Sas4^cKO;Trp53 ^−/− N = 4; one‐way ANOVA with post hoc analysis.
• F, G
  Quantification of the number of cells in the (F) superficial layer (CUX1⁺) and (G) deep layer (CTIP2⁺) within a 500 μm‐width column of P14 cortices. WT littermates N = 3, Sas4^cKO N = 3, Sas4^cKO;Trp53bp1 ^−/− N = 4, Sas4^cKO;Usp28^cKO N = 4, Sas4^cKO;Trp53 ^−/− N = 4; one‐way ANOVA with post hoc analysis.
• H
  P14 cortices of the indicated genotypes stained with antibodies against the deep layer marker CTIP2 (green), superficial layer marker CUX1 (red) and DAPI (blue). Scale bar = 200 μm.

Data information: All data represent the means ± SEM. *P < 0.05; **< 0.01; ****< 0.0001 and not significant indicates P > 0.05.