Figure 4. Inactivation of the mitotic surveillance pathway prevents NPCs depletion in brains with centrosome defects.

• A
  Cortices from E14.5 brains stained with antibodies against the radial glial cell marker PAX6 (yellow), intermediate progenitor marker TBR2 (red) and DAPI (blue). Scale bar = 50 μm.
• B, C
  Quantification of the number of (B) radial glial cells (PAX6⁺) and (C) intermediate progenitors (TBR2⁺) within a 250 μm‐width column of E14.5 cortices. WT N = 3, Cep63^T/T N = 4, Cep63^T/T;Usp28 ^−/− N = 3, Sas4^cKO N = 3, Sas4^cKO;Usp28^cKO N = 4; one‐way ANOVA with post hoc analysis.
• D
  Cortices from E14.5 embryos stained with antibodies against cleaved‐caspase 3 (CC3, white), TP53 (red) and DAPI. Scale bar = 50 μm.
• E, F
  Quantification of the number of CC3⁺ apoptotic cells (E) and cells with TP53 activation (F) within a 250 μm‐width column of E14.5 cortices. WT N = 5, Cep63^T/T N = 3, Cep63^T/T;Usp28 ^−/− N = 3, Sas4^cKO N = 4, Sas4^cKO;Usp28^cKO N = 4; one‐way ANOVA with post hoc analysis.

Data information: All data represent the means ± SEM. *P < 0.05; **< 0.01; ***< 0.001; ****< 0.0001 and not significant indicates P > 0.05. See also Fig EV4.