Figure EV6. The effect of nine TR knockdown on network integrity.

1. Shown is a schematic representation of the knockdown (KD) effects of each of the indicated TRs on the expression of the other factors and on network integrity, as determined by RT–qPCR analyses. Dark blue color denotes TRs upregulated more than twofold. Reduction by more than 2 fold in the expression is depicted by the absence of the corresponding TR. The light red and blue colored circles indicate downregulation or upregulation in the range of 1.3 to twofold, respectively, while gray circles indicate no change in gene expression. O: Oct4, S: Sox2, K: Klf4, and M: c‐Myc. The light gray‐green and red boxes depict the upstream and downstream layers of the 9TR GRN. The first panel on the left denotes the native 9TR GRN as shown in Fig 4B (phase III).
2. Same as in (A) except that double KDs of the indicated TRs were carried out.

Source data are available online for this figure.