Table 1.

PCR primers utilized for determination of parapoxvirus genus and pseudocowpox species.

Parapoxvirus primer set3 • PPVF: 5’ TCG ATG CGG TGC AGC AC 3’ • PPVR: 5’ GCG GCG TAT TCT TCT CGG AC 3’ • PPVP: 5’ FAM-TGC GGT AGA AGC C-MGB 3’

qORF primer set4 • qorfF: 5’ CAG CAG AGC CGC GTG AA 3’ • qorfR: 5’ CAT GAA CCG CTA CAA CAC CTT CT 3’ • qorfP: 5’ FAM-CAC CTT CGG CTC CAC-MGB 3’

Pan-parapoxvirus primer set5 • PPP1: 5′ GTC GTC CAC GAT GAG CAG CT 3′ • PPP4: 5′ GCG AGT CCG AGA AGA ATA CG 3′

PCR: polymerase chain reaction; qPCR: qualitative polymerase chain reaction.