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Quantification of Annexin V-negative and 7-ADD-positive AEC after CyaA and CyaA-AC– incubation. The percentage of Annexin V-positive and 7-ADD-negative cells is shown for HNEC (a), HTEC (b) and HBEC3-KT (c) after incubation with 0.1, 0.5, 1.0 µg/ml or 5.0 μg/ml of CyaA or CyaA-AC– for 24 h. Data are presented as means (bars)+SEM (error bars) of three independent experiments. Statistically significant differences compared to the control group (TUC) are indicated (*P < 0.05, ANOVA).
