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. 2020 Dec 14;27(1):89–98. doi: 10.1177/1753425920979354

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© The Author(s) 2020

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — CyaA affects intracellular cAMP concentration and IL-6 secretion in human airway epithelial cells. (a) HNEC, HTEC and HBEC3-KT were incubated with CyaA or CyaA-AC^– and intracellular cAMP concentration was analysed (n = 4). (b) Quantification of human IL-6 secreted by HNEC, HTEC and HBEC3-KT populations after treatment with CyaA or CyaA-AC^– was assayed by ELISA of the supernatants (n = 3). (c) Quantification of human IL-6 secreted by human nasal mucosa tissue models after treatment with CyaA or CyaA-AC^– (n=3). All data are presented as means (bars) + SEM (error bars). Differences after CyaA incubation compared to the control group (TUC) are indicated (*P ≤ 0.05, ^$P > 0.05 ≤ 0.1, ANOVA). Statistically significant differences of IL-6 basal levels are indicated (^#P < 0.05, ANOVA). Significant cell type-specific differences are indicated (^&P < 0.05, ANOVA).