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. 2021 Jan 4;23:1. doi: 10.1186/s12575-020-00137-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — The procedures for the completely-closed orthotopic transplantation of gastric cancer in NSG mice. A. a. Skin incision made using iris scissors. b. Exposing the stomach. Retractors hold both the skin and abdominal walls while forceps gently grab the stomach. B. A syringe assembly using an 18 gauge blunt needle and filled with homogenous minced tumor tissue. C. a. Injection of a low concentration of matrigel. b. Widening the entry to the space created under the serosa layer. c. Transplantation of up to 20ul of homogenous minced tissue. d. Tumor completely loaded into the stomach wall without leakage. D. a. Injection of a low concentration of matrigel. b. Widening the entry to the space created under the serosa layer. c. Transplantation of a 3 mm diameter fragment of soft tumor d. The tumor tissue completely inserted into the stomach wall without exposure. E. a. The abdominal wall closure with suture. b. The skin closed with wound clips. F. An illustration of the properly positioned transplantation site of the completely closed implantation method