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. 2021 Jan 4;23:1. doi: 10.1186/s12575-020-00137-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — The procedure to transplant the homogeneous tissues under the stomach serosa layer. A. The layers compose the stomach wall. B. Using a 31 gauge needle on an insulin syringe 30–40 ul of either 50% matrigel diluted with RPMI media or sterile saline is injected into the subserosa area. C. The serosa over the injection site is incised 2–3 mm with Vannas Iridocapsulotomy Scissors in order to generate the entrance to insert the 18 gauge need and evacuate the space-creating 50% matrigel or saline. D. The solution which had leaked is wiped with sterile gauze or cotton swabs. E. A cutting site is widened using Micro-Blunted Tip Dumont Forceps or Moria Iris Forceps. F. An 18 gauge needle is placed into the inner-space. G. Fill the tissue slowly from the opposite area of the entrance. H. After implantation, the entry site is pressed lightly with a sterile cotton swab and the needle is gently removed. I. Giving an extremely light and gentle pressure the transplantation site to confirm the leaking and the entry site is pressed with a sterile cotton swab for 20–30 s, on average, until it closes completely. J. As an auxiliary closure, tissue adhesive is beneficial to cover the incision site of the stomach serosa