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. 2020 Oct 6;2(2):fcaa165. doi: 10.1093/braincomms/fcaa165

Figure 2.

Figure 2

HFCD diet induces neuroinflammation at short-term. Mice were fed a control (n = 4–7 for each time point) or HFCD diet (n = 3–7 for each time point) for 14 and 24 weeks. The total RNA from cortex and hippocampus regions were extracted for qRT-PCR analysis, as described in Materials and methods section. Expression levels of pro-inflammatory markers, including IL-1β, TNF-α, Nlrp3 and Tlr4 in the hippocampus (A) and cortex (B). Hprt was used as loading control. Data are expressed as fold change over control. Data represent mean values ± SEM for at least five individual mice. *P < 0.05 and **P < 0.01 compared to control.