Skip to main content
. 2020 Oct 6;2(2):fcaa165. doi: 10.1093/braincomms/fcaa165

Figure 5.

Figure 5

HFCD diet promotes SCFA metabolic pathways. Mice were fed a control (n = 5 for each time point) or HFCD diet (n = 8–10 for each time point) for 14 and 24 weeks. Luminal samples of small intestine and cecum were collected as described in Materials and methods section. The impact of HFCD diet on the metabolism of gut microbiota was predicted by LDA analysis in both feeding time points. The pathway of KEGG was predicted by LDA coupled with effect size measurements in the small intestine after 14 weeks (A) and in the cecum after 24 weeks (B). The most differentially abundant pathways enriched by HFCD diet and ND were indicated in red and green, respectively. Arrows mark enriched SCFA metabolic pathway. Only pathways meeting an LDA significant threshold of >2 were shown. Validation of propionate pathway and measurement of propionate concentration in the liver of the ND- and HFCD-fed mice by LC-MS/MS (C). Data represent mean values ± SEM for four individual mice. *P < 0.05 compared to control.