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. 2020 Oct 6;2(2):fcaa165. doi: 10.1093/braincomms/fcaa165

Figure 8.

Figure 8

Dietary changes induce mitochondrial biogenesis and oxidative state in adult neurogenic niches. Mice were fed a control (n = 4–5 for each time point) or HFCD diet (n = 4–7 for each time point) for 14 and 24 weeks. Total RNA from DG- and OB-enriched extracts were processed for qRT-PCR, and brain slices were immunostained, as described in Materials and methods section. Effect of HFCD diet on Tfam (A, left), Sirt3 (B) and PGC-1α (C, left) mRNA levels in enriched extracts derived from the DG and OB. Hprt was used as loading control. Data are expressed as fold change over control. Representative images of immunofluorescence detection of DG and SVZ labelled with anti-Tfam (A, right) and anti-PGC-1α (C, right) antibodies. Nuclei were stained with Hoechst 33258. Scale bar, 5 μm. Data represent mean values ± SEM for at least five individual mice. *P < 0.05, **P < 0.01 and ***P < 0.001 compared to control.