Fig. 5.

Trade-offs among genome size, growth rate, and growth condition. a Growth rates relative to the wild type strain BY4742. The growth curves of ZLY348, ZLY298, and BY4742 were measured and maximum growth rates were calculated as we described previously [30]. The growth rate of BY4742 was normalized to 1 and the growth rates of ZLY348 and ZLY298 were shown as mean ± SD. Six replicates for each strain were used to calculate the mean and standard deviation. b Tenfold serial dilution was performed for ZLY348 and synXIIL under indicated conditions, and plates were imaged at the indicated time after plating. c Tenfold serial dilution was performed for indicated strains under conditions identical to b and plates were imaged at the indicated time after plating. d PML1, but not MEU1, is required for high temperature tolerance. Tenfold serial dilution was performed for these strains onto the indicated medium, and plates were imaged at the indicated time after plating. e SDH2, but not VPS13, is required for the utilization of glycerol as carbon source. Tenfold serial dilution was performed for these strains onto the indicated medium, and plates were imaged at the indicated time after plating