Fig. 6.

CircNOL10 regulates SOCS2 expression by sponging miR-767-5p in BC cells. a The potential candidate targets of miR-767-5p predicted by TargetScan, miRDB and DIANA-microT were further screened to search the down-regulated genes in BRCA by GEPIA database according to the criteria of log2 fold change < −2. b The expression difference of SOCS2 in 1085 BRCA tumor tissues and 291 normal tissues from GEPIA database. c The survival curves of BC patients with high or low expression of SOCS2 based on Kaplan–Meier plotter platform. d Diagram of the complementary binding sites between SOCS2 and miR-767-5p, as well as the mutant sequences of SOCS2 on miR-767-5p. e The relative luciferase activities of wild-type and mutant SOCS2 reporters in BT-549 and MDA-MB-231 cells transfected with miR-NC, miR-767-5p, miR-767-5p + Vector or miR-767-5p + circNOL10 were assessed via dual-luciferase reporter assay. f RNA pull-down assay was performed in BT-549 and MDA-MB-231 cells by using biotin-NC or biotin-miR-765-5p. g The effect of miR-767-5p overexpression on SOCS2 protein expression in BT-549 and MDA-MB-231 cells was examined by western blot assay. h The protein level of SOCS2, JAK2, p-JAK2, STAT5, and p-STAT5 was detected via western blot assay in BT-549 and MDA-MB-231 cells after transfection with vector, circNOL10 or circNOL10 + miR-767-5p. i qRT-PCR was used to monitor the level of SOCS2 mRNA in tumor tissues and adjacent non-cancerous samples from 60 BC patients. j, k The correlation between SOCS2 and miR-767-5p or circNOL10 was analyzed by Pearson test. ***P < 0.001