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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Neuroscience. 2020 Nov 24;452:247–264. doi: 10.1016/j.neuroscience.2020.11.013

Fig. 9. Simultaneous, but not individual, loss of Lmx1a and Lmx1b prevents the development of excitatory neurons in cochlear and vestibular nuclei.

Fig. 9.

Pax6 (B-E), Tbr1 (G-J), and Atoh1 (M-P’) immunostained transverse sections of the hindbrain at indicated stages. High magnification panels correspond to regions boxed in adjacent diagrams (A, F, K, L) or low magnification panels.

(A-E) Numerous Pax6+ neurons were present in the cochlear nuclei (CN) of wild type control, Lmx1a KO, and Lmx1b KO mutants (arrowheads) but were not detected in Lmx1a/b DKO embryos.

(F-J) Tbr1+ neurons were present in the superior vestibular nuclei (SuVe) of wild type control, Lmx1a KO, and Lmx1b KO mutants (arrowheads) but were not detected in Lmx1a/b DKO embryos.

(K, L) Diagrams illustrating location of sections showing lower RL in control and Lmx1a/b mutant embryos (M-P). Sections were taken at the level of otic vesicles (ov, blue dashed line in whole mount diagrams in K, L). Tissue morphology is illustrated in right diagrams in K and L. (M-P’) Numerous Atoh1+ progenitors were present in the lower RL of wild type control, Lmx1a KO and Lmx1b KO mutants (arrowheads) but not in Lmx1a/b DKO embryos.

Scale bar: 100 μm.