Fig. 6.

Biological validation of the antigenicity of neoantigen candidates in clinical samples. a Experimental scheme of immunization. HLA-A24 Tg mice were vaccinated with neoantigen candidate peptides three times (at days 0, 7, and 14). One week after the last immunization, mice were euthanized and isolated splenocytes were used for IFN-γ ELISpot assay. b The results of the ELISpot assay. c Statistical analysis of the results of ELISpot. Dark gray and light gray bars indicate independent mice experiments. *P < 0.05 (Student’s t test) and signal-to-noise ratio > 5. Error bars represent the SEM