Fig. 7.

Clinical relevance of our aberrant isoform catalogs in TCGA. a–d The number of isoforms calculated from the TCGA short-read RNA sequencing dataset (left panel), somatic mutation patterns of genes included in the NMD factors (right panel), and heatmaps showing the GSVA enrichment scores of the gene sets of the gene ontology biological process in lung adenocarcinoma (a), lung squamous cell carcinoma (b), colon adenocarcinoma (c), and pancreatic adenocarcinoma (d). Blue bars in the left panels represent specimens with damaging mutations in the NMD factors and green lines represent the median of isoforms. The P value was calculated using the one-tailed Mann–Whitney U test for testing the positive association of NMD factor mutations with a higher number of isoforms