Figure 2.

Proteotoxic stressors elicit multiple adaptive regimes. (A) Measurements of fold degron stability (top row, solid: Cyto-Deg; dotted: ERm-Deg) and fold PSR activity (bottom row) in titrations of bortezomib, canavanine, or AZC. (B) Measurement of PSR activity (right) after treatment with 5 µM bortezomib and either 200 µM canavanine, 1 mM AZC, or no additional treatment, at the noted time points (left). (C) Schematic of adaptive regimes as a function of degron stability and PSR activity. (D) Plots of fold degron stability (left: Cyto-Deg; right: ERm-Deg) versus fold PSR activity for the titrations in A to reveal the adaptive regime for each stressor. The boxed regions in the upper plots are enlarged in the lower plots. Error bars denote standard error for n ≥ 3 biological replicates. can, canavanine.