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. 2020 Dec 31;220(3):e201912041. doi: 10.1083/jcb.201912041

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© 2020 Work and Brandman

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S1. — Folding stressors increase RPN4 mRNA in wild type but not in the pYEF3:RPN4 background. (A and B) Mean fold RPN4 mRNA measured by RT-qPCR in wild-type yeast (A) or pYEF3:RPN4 yeast (B) after treatment with canavanine, AZC, or bortezomib. Values are normalized to ACT1 mRNA, then normalized to no treatment. Significance between the untreated and treated samples was determined by a paired two-sided Student’s t test. Error bars denote standard error for n = 3 biological replicates. ns, P > 0.05; *, P < 0.05; and **, P < 0.01. btz, bortezomib; can, canavanine; treat, treatment.