Figure S3.

Folding stressors cause aggregation and recruitment of heat shock proteins. (A) Fluorescent localization of ERm-Deg (GFP), Cyto-Deg (GFP), or Hsp104-mKate2 (RFP) in cells treated with 200 µM canavanine, 1 mM AZC, 40 µM bortezomib, or no treatment for 5 h. Cells are outlined in yellow dashed lines. (B) Immunoblot of Cyto-Deg after cells were treated with canavanine, AZC, or bortezomib. Aggregated material was collected from the cells by centrifugation, and the input, supernatant (sup), and pellet were immunoblotted. Arrows denote the position of Cyto-Deg. (C) Quantification of n = 3 biological replicates of the immunoblot in B. Values are normalized to the respective input of each sample. (D) Fluorescent localization of GFP-Sis1 and GFP-Ssa1 in cells treated with 800 µM canavanine, 4 mM AZC, 40 µM bortezomib, or no treatment for 5 h. btz, bortezomib; can, canavanine; Exp., exposure; treat, treatment.