Figure S5.

Short-lived proteins Nce103 and Ctk1 under folding and proteolytic stressors. (A) Measurements of fold protein (top: GFP-Nce103 and GFP-Tdh3; bottom: GFP-Ctk1 and GFP-Tdh3) levels in titrations of bortezomib, canavanine, or AZC. Significance was collectively tested across the three highest concentrations measured by combining data from these concentrations into one group per genetic context, then performing a paired two-sided Student’s t test. Error bars denote standard error for n > 3 biological replicates. (B) Fluorescent localization of GFP-Nce103, GFP-Ctk1, GFP-Tdh3, and free GFP in cells treated with 800 µM canavanine, 4 mM AZC, 40 µM bortezomib, or no treatment for 5 h. Free GFP is expressed from pTDH3. Cells are outlined in yellow dashed lines. **, P < 0.01; and ***, P < 0.001. btz, bortezomib; can, canavanine.