Table I.
Description of the 8 miRNA primers used for reverse transcription-quantitative PCR.
| miRNA primer | Function | Sequence |
|---|---|---|
| UniSp2 | RNA extraction efficiency | Unavailable |
| cel-miR-39-3p | cDNA synthesis efficiency | 5'-UCACCGGGUGUAAAUCAGCUUG-3' |
| hsa-miR-451a | Stably expressed miRNA of interest and detection of haemolysis | 5'-AAACCGUUACCAUUACUGAGUU-3' |
| hsa-miR-23a | Stably expressed miRNA of interest and detection of haemolysis | 5'-AUCACAUUGCCAGGGAUUUCC-3' |
| hsa-let-7i-3p | Stably expressed miRNA used for normalisation | 5'-CUGCGCAAGCUACUGCCUUGCU-3' |
| hsa-miR-148-3p | Stably expressed miRNA used for normalisation | 5'-UCAGUGCAUCACAGAACUUUGU-3' |
| hsa-miR-222-3p | Stably expressed miRNA of interest | 5'-AGCUACAUCUGGCUACUGGGU-3' |
| hsa-miR-30e-5p | Stably expressed miRNA of interest | 5'-UGUAAACAUCCUUGACUGGAAG-3' |