Table II.

Method of identifying RT-qPCR data outliers^a.

Mean Cq of replicates	Maximal acceptable variance in Cq replicates
25	0.5
26	0.5
27	0.5
28	0.5
29	0.5
30	0.5
31	0.5
32	0.7
33	0.9
34	1.3
35	1.9

^aFor each miRNA replicate, the mean Cq value and variance was calculated and outliers removed accordingly. RT-qPCR efficiency was calculated to be 2.0. RT-qPCR, reverse transcription-quantitative PCR.