Table 2. Schedule protocol of PCR for rs2305948.
| Kind of cycle | temperature | time | Cycle number |
|---|---|---|---|
| Initial Denaturation | 95 C | 5 minutes | 1 |
| denaturation | 95C | 1 minute | |
| Annealing | 58 C | 1 minute | 30 |
| Extension | 72 C | 1 minute | 1 |
| The final Extension | 72 C | 10 minutes | 1 |
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