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SDS-PAGE analysis of expression of the fusion protein. Lane marker: spectrum multicolor broad-range protein ladder. Lanes 1~3: whole-cell extract of 3 E. coli hosts under induced conditions in the presence of 10 mM DTT. Lanes 4~6: whole-cell extract of 3 E. coli hosts under non-induced conditions in the absence of DTT. Lane 1 and 4: Tuner (DE3); Lanes 2 and 5:BL21 (DE3); Lanes 3 and 6: Rosetta 2 (Gami).
