Figure 3.

EC-specific WWOX KO mice exhibit greater vascular leak during LPS-induced ARDS. Six WWOX flox (control) and six WWOX KO mice were treated with intratracheal instillation of either PBS or 1 mg/kg LPS (n = 3 per group). Eighteen hours later, all mice underwent BAL using 1 ml of PBS. Lungs were then harvested for Western blotting and histologic examination. (A, B, and D) Bar graphs depict BAL leukocyte counts, protein concentration, and FITC dextran flux as a measure of permeability. Results are shown as means ± SD in n = 3 independent experiments. *P < 0.05, compared with LPS-treated control by Student’s t test. (C) A representative hematoxylin and eosin–stained lung histologic section from each of the four experimental conditions is shown here.