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. 2020 Dec 31;15:3407–3416. doi: 10.2147/COPD.S278553

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© 2020 Liu et al.

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MiR-218 overexpression weakened apoptosis and inflammation in CSE-induced BEAS-2B cells. (A and B) The relative expression level of miR-218 was evaluated by RT-qPCR in BEAS-2B cells treated with different concentrations of CSE (0%, 1%, 2%, 4%, and 8%) for 24 h or treated with 2% CSE for different times (0 h, 12 h, 24 h, 36 h, and 48 h). (C) The expression of miR-218 was assessed by RT-qPCR assay in CSE-induced BEAS-2B cells transfected with miR-218 or miR-NC. (D–F) The levels of IL-6, IL-8, and TNF-α were measured by ELISA kit in the supernatants. (G) Apoptotic cells were monitored by ﬂow cytometry assay. Data are shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.

Abbreviation: CSE, Cigarette smoke extract.