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[Preprint]. 2020 Dec 23:2020.12.22.422708. [Version 1] doi: 10.1101/2020.12.22.422708

Fig. 2. Screening array of GST-PDZ domain fusion proteins.

Fig. 2.

The indicated GST-PDZ and GST-14-3-3 domain fusion proteins per lettered box were arrayed in duplicate as shown. The bottom right sample (M) in each box represents GST alone as a negative control. The array was screened with biotinylated E-WT or E-mutant peptides of SARS-CoV-2 E protein. Representative data for E-WT peptide (Biotin-SRVKNLNSSRVPDLLV) (100μg), and E-mutant peptide (Biotin-SRVKNLNSSRVPAAAA) (100μg) are shown (bottom panels). The E-mutant peptide did not interact with any GST-PDZ or GST-14-3-3 domain fusion proteins, whereas the E-WT peptide interacted strongly and solely with GST-PDZ domain #2 from human ZO1 in position 6 in box J (red oval). A positive control for expression of all GST fusion proteins is shown (anti-GST).