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. 2021 Jan 4;16(1):e0244513. doi: 10.1371/journal.pone.0244513

Fig 3. 3D spheroids show decreased apoptosis rates after irradiation compared with 2D cultures.

Fig 3

(A) CRC cells were irradiated with 1, 4, and 10 Gy. Six days after irradiation, cells were stained with annexin V/ PI, and analysed by flow cytometry. Significance was calculated using a two-sided, unpaired Student’s T test. (B) CaCo2 and HCT116 cells were irradiated with 0 and 1 Gy. Six days after irradiation, cells were fixed with 4% PFA, embedded in paraffin and stained for TUNEL assay. Significance was calculated using a two-sided, unpaired Student’s T test. (C) Representative TUNEL stainings for CaCo2 and HCT116 2D and 3D cultures six days after irradiation with 0 and 1 Gy. Scale bar 50 μm.