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. 2021 Jan 4;16(1):e0244856. doi: 10.1371/journal.pone.0244856

Table 2. qRT-PCR validation of the RNA-seq results.

Gene ID Function annotations RNA-seq qRT-PCR Regulation
Fold change FDR Fold change p-value
gene-EREB60 AP2-EREBP transcription factor 2.74 6.12E-04 2.28 2.16E-03 up
gene-LOC541743 Transcription factor MYB30 isoform X1 3.51 4.87E-03 5.58 2.67E-06 up
gene-MYB64 Transcription repressor MYB6 2.48 1.42E-06 5.32 3.12E-03 up
gene-myb8 Transcription factor MYB8 3.83 5.45E-12 6.26 2.66E-02 up
gene-LOC103651266 WRKY transcription factor 51 3.74 2.39E-05 2.18 1.89E-03 up
gene-WRKY45 WRKY DNA-binding domain superfamily protein 0.37 3.91E-04 0.25 3.06E-04 down
gene-LOC103628959 MADS-box transcription factor 26 0.30 1.75E-04 0.38 8.46E-02 down
gene-bHLH94 bHLH DNA-binding domain superfamily protein 2.33 1.88E-04 4.37 4.56E-03 up
gene-LOC103631852 Transcription factor EMB1444 2.22 4.93E-04 3.89 2.49E-05 up
gene-LOC103633674 NAC transcription factor 32 8.55 2.85E-04 6.26 1.80E-03 up
gene-LOC103653847 NAC domain-containing protein 7 0.20 6.75E-03 0.13 5.17E-03 down
gene-LOC103625838 Transcription factor JUNGBRUNNEN 1 2.46 3.63E-03 3.36 4.39E-02 up
gene-LOC103630375 Ethylene-responsive transcription factor ERF020 0.36 9.69E-03 0.29 3.68E-05 down
gene-LOC103635988 Ethylene-responsive transcription factor WRI1 2.86 2.45E-04 4.16 6.44E-03 up
gene-GLN6 Glutamine synthetase root isozyme 1 2.88 2.34E-07 2.65 3.32E-02 up
gene-nrt2 Ammonium transport 2 3.42 3.66E-03 3.56 1.26E-04 up
gene-nrt2.2 High affinity ammonium transporter 3.14 7.32E-04 3.68 1.86E-03 up
gene-HAK20 Potassium transporter 5 3.00 8.11E-03 3.22 3.36E-03 up
gene-HAK21 Potassium transporter 21-like isoform X1 4.87 4.65E-15 5.56 3.72E-03 up
gene-kup1 Potassium ion uptake permease 1 3.06 9.09E-09 3.26 4.70E-03 up