Figure 2.

Comparison of viability and purity after the different separation techniques

Viability was determined with 7-AAD staining while purity was expressed as a percentage of CD4⁺ cells in the living cell population (see Methods). Measurements were made via flow cytometry, subsequently after the separation (values are plotted as mean±SD for the number of independent experiments indicated in the bar). No significant differences were found among the groups for either viability or purity (one-way analysis of variance, p > 0.05). REAlease®: measured after the complete removal of bead-antibody and REAlease® complex, in label-free configuration (see Figure 1), Negative selection: immunomagnetic negative selection, Sorted: positive selection by flow cytometry.